The mission of the Kolker Lab is to propel biomedical discovery by translating Data to Knowledge to Outcomes. We accelerate and optimize your work through intuitive, reliable, and powerful analytics.
Data to Knowledge to Outcomes
- Principles of Proteomics
- Software Tools for Proteomics Analysis:
- Endothelial Cells for BCBC
Basic Principles of Proteomics:
Mass Spectrometry and Column Chromatography Explained
Our goal for K-12 outreach is to provide a basic understanding of proteomics through hands-on activities.
Read MoreMOPED and SPIRE
SPIRE is a freely available, web-based proteomics analysis pipeline, which researchers use to process raw mass spectrometry data into protein identifications and analysis options. MOPED is a proteomics database that supports rapid browsing of protein expression and meta-analysis capabilities for model organisms and humans.
Read MoreIdentifying Proteins
Related to Beta Cell Development (BCBC – Ken Zaret)
Deficiencies in beta cell (insulin producing cells) development are key to type 1 diabetes and other diseases. Our objective for this project is to use MS proteomics to identify proteins related to beta-cell development from endothelial cells.
This project is challenging because the secreted proteins of interest are very low in abundance and assays to detect activity are very costly and time consuming.